However, the natural history of zebrafish remains poorly understood, and the specific functions of pigmentation have yet to be addressed in the wild. The zebrafish genome encodes two paralogs, kita and kitb, following genome. Gene duplication of the zebrafish kit ligand and partitioning of melanocyte development functions to kit ligand a keith a. Moreover, they characterize a new zebrafish cytokine, oncostatin m osm, which acts upstream of kit signaling during hsc specification but synergizes with kitlgb to promote hsc expansion in the caudal hematopoietic tissue. Here we show that bnc2 promotes melanophore and xanthophore development by regulating expression of the growth factors kit ligand a kitlga and colony. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. After searching the zebrafish genetic trace database for a kitl ortholog using protein sequence from multiple species, we identified two partial candidate genes on. Hematopoietic cytokine gene duplication in zebrafish.
The zebrafish, danio rerio, owing its name to the striking stereotypic pattern of longitudinal blue and yellow stripes, has emerged as the model organism for colour pattern formation in vertebrates. Citeseerx document details isaac councill, lee giles, pradeep teregowda. In the zebrafish, several lines of direct and indirect evidence in our. Scf stem cell factor, also known as c kit ligand kl, mast cell growth factor mgf, and steel factor slf promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. Granulins regulate aging kinetics in the adult zebrafish.
During oocyte maturation, kit ligand expression in the granulosa cells shifts from the membranebound to the soluble form and both forms are lost in the cumulus cells ismail et al. In zebrafish, the role of kit in hematopoiesis still remains unclear. Please use this form to recommend updates to the information in zfin. Is expressed in several structures, including endocrine system. Bertrand1, 1university of geneva, faculty of medicine, department of pathology and immunology, cmu, university of geneva, 1 rue michelservet, geneva 1211, switzerland. Kit signaling is required for development of coordinated. Johnsongene duplication of the zebrafish kit ligand and partitioning of melanocyte development functions to kit ligand a. Oncostatin m and kit ligand control hematopoietic stem cell fate during. We examine the partitioning of expression patterns and functions of two zebrafish kit ligands, kit ligand a kitla and kit ligand. Citeseerx gene duplication of the zebrafish kit ligand. In the zebrafish, several lines of direct and indirect evidence in our previous reports also suggested a correlation between kitlga and oocyte maturation. The kit system, including kit ligand and its receptor kit, has critical roles in mammalian reproduction, especially in the ovary.
In mouse testis, kit ligand and its receptor is expressed in sertoli cells during. Pdf gene duplication of the zebrafish kit ligand and. Interactions with iridophores and the tissue environment required for patterning melanophores and xanthophores during zebrafish adult pigment stripe formation. Human skin color and fish genes understanding genetics. Kit signaling is involved in melanocyte stem cell fate in zebrafish embryos article in development 1405 january 20 with 11 reads how we measure reads. A guide for the laboratory use of zebrafish danio rerio eugene oregon.
In contrast to mammals, zebrafish have two orthologs of grn, granulin a grna and granulin b. Gene duplication of the zebrafish kit ligand and partitioning. We examine the partitioning of expression patterns and functions of two zebrafish kit ligands, kit ligand a kitla and kit ligand b kitlb, and. In mammals, kit ligand is known to inhibit gvbd or the resumption of meiosis. Although several studies have described early lymphatic development in the fish, lymphatic development at later stages has not been completely elucidated. Zebrafish stripes as a model for vertebrate colour pattern. Ligandassisted reconstruction of colloidal quantum dots. The retention of particular genes after the whole genome duplication in zebrafish has given insights into how genes may evolve through partitioning of ancestral functions. Phylogenetic and chromosome synteny analyses suggest that these duplicated genes. Moreover, they characterize a new zebrafish cytokine, oncostatin m osm, which acts upstream of kit signaling during hsc specification but synergizes with kitlgb to promote hsc expansion in the caudal. This report demonstrates the duplication of the kit ligand gene in zebrafish and shows that expression and function specific to the development of the melanocyte have been partitioned to one of these ligands. Johnson1 1 department of genetics, washington university school of medicine, saint louis, missouri, united states of america, 2 department of molecular genetics and biochemistry.
Kit signaling is involved in melanocyte stem cell fate in. Diseases associated with kitlg include hyperpigmentation with or without hypopigmentation, familial progressive and deafness, autosomal dominant 69. Human orthologs of this gene implicated in autosomal dominant nonsyndromic deafness 69. Development of the larval lymphatic system in zebrafish. One such coorthologous pair is the zebrafish kit genes,14.
Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. As expression of the kit protein is correlated with icc in other species, the expression of the kit protein in zebrafish indicates the presence of icc in the zebrafish gi tract. Oncostatin m and kitligand control hematopoietic stem cell fate during. We appreciate as much detail as possible and references as appropriate. Evidence for functional divergence of two isoforms of kit kita and kitb and kit ligand kitlga and kitlgb. The presence of hydroxyl groups on the nanoparticle surface, as well as dimersfusion between cqdshas been found to be the.
A guide for the laboratory use of zebrafish brachydanio rerio. Kit signaling plays an important role in a variety of tissues. In this paper, bertrand and colleagues show that kit signaling is necessary to the development of zebrafish hematopoietic stem cells, as well as for their expansion. This gene encodes the ligand of the tyrosinekinase receptor encoded by the kit locus. Gene duplication of the zebrafish kit ligand and partitioning of melanocyte development functions to kit ligand a. Expression of mrna encoding for the two zebrafish orthologues of mammalian kit, kita, and kitb, as well as two orthologues for mammalian kit ligand, kitla and kitlb, was confirmed in adult zebrafish gi tissues. Increasing the power conversion efficiency pce of colloidal quantum dot cqd solar cells has relied on improving the passivation of cqd surfaces, enhancing cqd coupling and charge transport, and advancing device architecture. Enrichment of hematopoietic stemprogenitor cells in the zebrafish. Kitlgscf binding can activate several signaling pathways. Stem cell factor also known as scf, kitligand, kl, or steel factor is a cytokine that binds to the ckit receptor cd117. Acknowledging materials and services please acknowledge the zebrafish international resource center in all publications resulting from the materials andor services we are providing to you. Pdf oncostatin m and kitligand control hematopoietic. Based on mutational analysis in zebrafish, we show that the fgf ligand 24 fgf24 is required for proliferation, differentiation, and morphogenesis of the early somatic gonad, and as a result. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa.
Oncostatin m and kitligand control hematopoietic stem. Stem cell reports article oncostatin m and kit ligand control hematopoietic stem cell fate during zebra. Total rna from the entire telencephalon was isolated with qiagen rneasy kit, whereas total rna from. Here, we investigate the bmp ligand gdf6a, a zebrafish ortholog of human gdf6. Thus, duplication of the kit ligand gene in zebrafish was followed by splice form degeneration similar to what has. Genetics and genomics reliable lab solutions on free shipping on qualified orders. Development takes place outside the mother, allowing direct inspection at all stages. Full text differential regulation of kit ligand a kitlga expression in the zebrafish ovarian follicle cells evidence for the existence of a cyclic adenosine 3. Regulation of zebrafish melanocyte development by ligand.
Fish were mated, raised and staged according to the zebrafish book. The zebrafish scyba gene encodes a novel cxctype chemokine with distinctive expression patterns in the vestibuloacoustic system during embryogenesis. Designed as a part of a larger commitment to the childrens hospital boston, the book stresses how individuals can make a difference locally and globally. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Oncostatin m and kit ligand control hematopoietic stem cell fate during zebrafish embryogenesis article pdf available in stem cell reports 106 may 2018 with 101 reads how we measure reads. Scf can exist both as a transmembrane protein and a soluble protein. Among its related pathways are ret signaling and downstream signaling events of b cell receptor bcr. Ligand for the receptortype proteintyrosine kinase kit. Spatial distribution and receptor specificity of zebrafish. Kit ligand kitlg is a classic example of a cytokine acting on many different levels. A guide for the laboratory use of zebrafish danio rerio. The existence of two copies of kit ligand, each corresponding to an alternative splice form of mouse and human, lead us to hypothesize that zebrafish kitla retains the function of the mammalian isoform 1 and that kitlb, having lost exon 6, would retain the function of mammalian isoform 2.
However, little is known about the role of kit signaling during the emergence of definitive hematopoiesis in the mouse embryo. The genetic mechanism of sd in zebrafish danio rerio, a widely used model organism. Interactions with iridophores and the tissue environment. The zebrafish is a powerful model organism that enables study of lymphatic vessel development using highresolution imaging and sophisticated genetic and experimental manipulation. Imperial tropicals 4 zebra nerite snails neritina natalensis great for algae and plant safe will not reproduce in fresh water. The zebrafish genome encodes two paralogs, kita and kitb, following genome duplication and possible subfunctionalization mellgren and johnson, 2005. We examine the partitioning of expression patterns and functions of two zebrafish kit ligands, kit ligand a kitla and kit ligand b kitlb, and discuss their possible coevolution with the duplicated. Laboratory studies of zebrafish suggest that pigmentation, and stripes in particular, facilitate social aggregation, or shoaling engeszer et al. In contrast to kit ligand kit in mammals and kitlgakita in the zebrafish, kitlgb was found to exist in the oocyte and kitb in the follicle layer, pointing to the possibility for a unique kitlgbkitb paracrine pathway in the zebrafish follicle that mediates the control of. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio by monte westerfield, institute of neuroscience, university of oregon older literature referred to zebrafish as brachydanio rerio. By far, the major advantage provided by zebrafish is the ease of generating transgenic. Reynolds is the first graphic novel from the popular fablevision publisher.
The books homepage helps you explore earths biggest bookstore without ever leaving the comfort of your couch. Regulation of teleost macrophage and neutrophil cell. Hultman ka, bahary n, zon li, johnson sl, 2007 gene duplication of the zebrafish kit ligand and partitioning of melanocyte development functions to kit ligand a. Commercial assay or kit, golden gate talen and tal effector kit 2. Stem cell factor also known as scf, kit ligand, kl, or steel factor is a cytokine that binds to the c kit receptor. Human skin color and fish genes europeans, east asians, and stickleback fish became lighter because of the kit ligand gene december 20, 2007 the color a species settles on partly depends upon its environment. Zebrafish kit ligands cooperate with erythropoietin to promote. This cytokine plays an important role in hematopoiesis formation of blood cells, spermatogenesis, and melanogenesis. Most importantly, the availability of mutants with altered patterns and feasibility of long. We were interested in whether the kitadependent pigment pattern in larval zebrafish required kit ligand kitl in mouse, kitlg in human, also referred to as mgf, scf. Here youll find current best sellers in books, new releases in books, deals in books, kindle. We examine the partitioning of expression patterns and functions of two zebrafish kit ligands, kit ligand a kitla and kit ligand b kitlb, and discuss their possible coevolution with the duplicated zebrafish kit. Thus, our purification method of hscs in the zebrafish kidney is useful. The present invention has demonstrated the presence of the zebrafish kit protein resulting from the expression of the kit gene in the zebrafish gi tract.
537 911 191 1169 1089 994 235 574 160 557 387 656 1351 678 244 1498 628 115 900 845 347 165 898 193 1046 670 429 809 1546 248 580 293 1368 1056 663 1386 1130 49 1296 979